Effect of metformin on transcriptome and adipokinome of adipocytes of local fat deposts of patients with ischemic heart disease

BACKGROUND : The information available to date on the effect of metformin transcriptome and secretory capacity adipocytes in human adipose tissue (AT) is scarce contradictory. study analyzed whether vitro modulates gene expression secretion adiponectin leptin AT subcutaneous (SAT), epicardial (EAT) perivascular (PVAT) localization patients with ischemic artery disease (IHD). AIM To assess at various concentrations (1 10 mmol / L) level genes their content subcutaneous, IHD. MATERIALS AND METHODS included 134 IHD indications for direct myocardial revascularization by coronary bypass grafting (CABG). During operation, biopsies SAT, EAT PVAT were obtained 3–5 gram, which served as a source adipocytes. Isolated cultured 24 hours without L). After day incubation, ADIPOQ LEP culture medium determined. Statistical processing was performed using GraphPad Prism 6 software package (GraphPad Software, La Jolla, CA, USA) Statistica software, 6.1 (Dell Inc., Round Rock, TX, USA). RESULTS characterized an imbalance adipokine system, manifested low high comparison SAT. Metformin increased its regardless location, while concentration had stronger compared L. multidirectional adipocytes, depended localization: both (10 L), drug reduced protein no significant In PVAT, concentration. CONCLUSION has EAT, able modulate activity, promising strategy maintaining balance adipokines AT, especially localization.